מבוא להיסטולוגיה של מחלות במודל עכברי (0491.3566)

The course is designed for graduate students. The course will describe different approaches in histological study: from tissue processing to basic histology and immunohistology of pathological features in the brain, spinal cord and muscles of mouse models. Furthermore, the course will introduce the basis of microscopy techniques, from light, fluorescent, confocal to EM and the advantages and disadvantages of each. During the course three mouse disease models will be described: multiple sclerosis (MS), brain tumors (gliomas) and Amyotrophic Lateral Sclerosis (ALS). Lectures will include an introduction to histology and describe different approaches to identify pathologic changes in the tissue. The course will describe the background of each disease and explain the mouse models available for studying it. Furthermore, pathological features in each model will be explained and students will learn the recommended histological and immunohistological approaches for monitoring disease progression. Markers of both neuronal and neuroinflammation (glia activation and immune cell infiltration) will be discussed. During the course the students will learn different techniques of tissue processing towards preparing brain or spinal cord samples for basic histology and immunohistology using chemical and specific dyes that can be observed by either light or fluorescent microscope. Special emphasis will be on co-labeling of different pathological disease features. 

Background:

Courses covers basic cell biology and biochemistry
 

Final score: Exam